How to fill holes in binary image

Hello buddy,

I know that some posts already exist regarding this problem, but I'm still struggling in a similar issue. I have a .avi file from which I extract, frame by frame, the animal pupil, but are present in the image these three reflecting blobs of light that makes the binary conversion weird. I don't know how to get rid off them.

Someone, could help me, pls?

Ps: just to let you know I had this code within a for loop

    image_frame = read (video,cnt);
    piel_1 = ~im2bw(image_frame,0.15);
    piel_2 = bwmorph(piel_1,'open');
    piel_3 = bwmorph(piel_2,'close');
    piel_4 = bwareaopen(piel_3,2000);
    piel = imfill(piel_4,'holes');

Answer 1

[KALYAN ACHARJYA]

"How to fill holes in binary image"

J = imerode( binary_image_name , SE ) ;
% chosse the structuring elment as per your requiremnet

For detail see here

[LORENZO GRILLI]

Thanks for your answer, but I'm not able to manage the issue with the function that you suggested, I'm naive to Matlab and in general to coding, so sorry if I have not understood your suggestion.

Maybe the problem resides at the edge of the missing part because the function imfill replace the holes surrounded by 1 and I do not have enough 1 in the missing part.

Now, I have extracted the three reflecting blobs, and I'm trying to add them to the matrix of my pupil but seems not affecting the image. Maybe something is wrong in my code, please could you help me?

mask = imbinarize(image_frame);
for k = 1:length(piel)
         if piel(k,:) + mask(k,:) > 1
             piel(k,:) = piel(k,:);
         end
         if piel(k,:) + mask(k,:)==0
             piel(k,:) = piel(k,:);
         end
         if piel(k,:) + mask(k,:)==1
             piel(k,:)=piel(k,:)+mask(k,:);
         end
end

[darova]

What does this part suppose to mean?

piel(k,:) = piel(k,:);

Answer 2

[Image Analyst]

To fill holes in a binary image:

bw = imfill(bw, 'holes');

[Muhammad Zeeshan Ahmed Khan]

This is not filling the hole in a binary image please guide

[Image Analyst]

OK, sure. Where is your image? You forgot to attach it. ?‍♂️

Are you sure your image is just larger than the display and there is a small path to the background that gets subsampled away when it goes to display it so that you don't see that path to the background and you think it's a hole when it's really not? So I can check for that you must attach your binary image in a PNG format file or a .mat file, NOT as a JPG image.