Fit One-Compartment Model to Individual PK Profile

Background

This example shows how to fit an individual's PK profile data to one-compartment model and estimate pharmacokinetic parameters.

Suppose you have drug plasma concentration data from an individual and want to estimate the volume of the central compartment and the clearance. Assume the drug concentration versus the time profile follows the monoexponential decline $C_{t} = C_{0} e^{- k_{e} t}$ , where $C_{t}$ is the drug concentration at time t, $C_{0}$ is the initial concentration, and $k_{e}$ is the elimination rate constant that depends on the clearance and volume of the central compartment $k_{e} = C l / V$ .

The synthetic data in this example was generated using the following model, parameters, and dose:

One-compartment model with bolus dosing and first-order elimination
Volume of the central compartment (Central) = 1.70 liter
Clearance parameter (Cl_Central) = 0.55 liter/hour
Constant error model
Bolus dose of 10 mg

Load Data and Visualize

The data is stored as a table with variables Time and Conc that represent the time course of the plasma concentration of an individual after an intravenous bolus administration measured at 13 different time points. The variable units for Time and Conc are hour and milligram/liter, respectively.

clear all
load('data15.mat')

plot(data.Time,data.Conc,'b+')
xlabel('Time (hour)');
ylabel('Drug Concentration (milligram/liter)');

Figure contains an axes. The axes contains an object of type line.

Convert to groupedData Format

Convert the data set to a groupedData object, which is the required data format for the fitting function sbiofit for later use. A groupedData object also lets you set independent variable and group variable names (if they exist). Set the units of the Time and Conc variables. The units are optional and only required for the UnitConversion feature, which automatically converts matching physical quantities to one consistent unit system.

gData = groupedData(data);
gData.Properties.VariableUnits = {'hour','milligram/liter'};
gData.Properties

ans = struct with fields:
                Description: ''
                   UserData: []
             DimensionNames: {'Row'  'Variables'}
              VariableNames: {'Time'  'Conc'}
       VariableDescriptions: {}
              VariableUnits: {'hour'  'milligram/liter'}
         VariableContinuity: []
                   RowNames: {}
           CustomProperties: [1x1 matlab.tabular.CustomProperties]
          GroupVariableName: ''
    IndependentVariableName: 'Time'

groupedData automatically set the name of the IndependentVariableName property to the Time variable of the data.

Construct a One-Compartment Model

Use the built-in PK library to construct a one-compartment model with bolus dosing and first-order elimination where the elimination rate depends on the clearance and volume of the central compartment. Use the configset object to turn on unit conversion.

pkmd                    = PKModelDesign;
pkc1                    = addCompartment(pkmd,'Central');
pkc1.DosingType         = 'Bolus';
pkc1.EliminationType    = 'linear-clearance';
pkc1.HasResponseVariable = true;
model                   = construct(pkmd);
configset               = getconfigset(model);
configset.CompileOptions.UnitConversion = true;

For details on creating compartmental PK models using the SimBiology® built-in library, see Create Pharmacokinetic Models.

Define Dosing

Define a single bolus dose of 10 milligram given at time = 0. For details on setting up different dosing schedules, see Doses in SimBiology Models.

dose                = sbiodose('dose');
dose.TargetName     = 'Drug_Central';
dose.StartTime      = 0;
dose.Amount         = 10;
dose.AmountUnits    = 'milligram';
dose.TimeUnits      = 'hour';

Map Response Data to the Corresponding Model Component

The data contains drug concentration data stored in the Conc variable. This data corresponds to the Drug_Central species in the model. Therefore, map the data to Drug_Central as follows.

responseMap = {'Drug_Central = Conc'};

Specify Parameters to Estimate

The parameters to fit in this model are the volume of the central compartment (Central) and the clearance rate (Cl_Central). In this case, specify log-transformation for these biological parameters since they are constrained to be positive. The estimatedInfo object lets you specify parameter transforms, initial values, and parameter bounds if needed.

paramsToEstimate    = {'log(Central)','log(Cl_Central)'};
estimatedParams     = estimatedInfo(paramsToEstimate,'InitialValue',[1 1],'Bounds',[1 5;0.5 2]);

Estimate Parameters

Now that you have defined one-compartment model, data to fit, mapped response data, parameters to estimate, and dosing, use sbiofit to estimate parameters. The default estimation function that sbiofit uses will change depending on which toolboxes are available. To see which function was used during fitting, check the EstimationFunction property of the corresponding results object.

fitConst = sbiofit(model,gData,responseMap,estimatedParams,dose);

Display Estimated Parameters and Plot Results

Notice the parameter estimates were not far off from the true values (1.70 and 0.55) that were used to generate the data. You may also try different error models to see if they could further improve the parameter estimates.

fitConst.ParameterEstimates

ans=2×4 table
         Name         Estimate    StandardError      Bounds  
    ______________    ________    _____________    __________

    {'Central'   }     1.6993       0.034821         1      5
    {'Cl_Central'}    0.53358        0.01968       0.5      2

s.Labels.XLabel     = 'Time (hour)';
s.Labels.YLabel     = 'Concentration (milligram/liter)';
plot(fitConst,'AxesStyle',s);

Figure contains an axes. The axes with title Group One group contains 2 objects of type line. These objects represent OBS1 (Conc), PRED1 (Central.Drug_Central).

Use Different Error Models

Try three other supported error models (proportional, combination of constant and proportional error models, and exponential).

fitProp = sbiofit(model,gData,responseMap,estimatedParams,dose,...
                      'ErrorModel','proportional');
fitExp  = sbiofit(model,gData,responseMap,estimatedParams,dose,...
                      'ErrorModel','exponential');
fitComb = sbiofit(model,gData,responseMap,estimatedParams,dose,...
                      'ErrorModel','combined');

Use Weights Instead of an Error Model

You can specify weights as a numeric matrix, where the number of columns corresponds to the number of responses. Setting all weights to 1 is equivalent to the constant error model.

weightsNumeric = ones(size(gData.Conc));
fitWeightsNumeric = sbiofit(model,gData,responseMap,estimatedParams,dose,'Weights',weightsNumeric);

Alternatively, you can use a function handle that accepts a vector of predicted response values and returns a vector of weights. In this example, use a function handle that is equivalent to the proportional error model.

weightsFunction = @(y) 1./y.^2;
fitWeightsFunction = sbiofit(model,gData,responseMap,estimatedParams,dose,'Weights',weightsFunction);

Compare Information Criteria for Model Selection

Compare the loglikelihood, AIC, and BIC values of each model to see which error model best fits the data. A larger likelihood value indicates the corresponding model fits the model better. For AIC and BIC, the smaller values are better.

allResults = [fitConst,fitWeightsNumeric,fitWeightsFunction,fitProp,fitExp,fitComb];
errorModelNames = {'constant error model','equal weights','proportional weights', ...
                   'proportional error model','exponential error model',...
                   'combined error model'};
LogLikelihood = [allResults.LogLikelihood]';
AIC = [allResults.AIC]';
BIC = [allResults.BIC]';
t = table(LogLikelihood,AIC,BIC);
t.Properties.RowNames = errorModelNames;
t

t=6×3 table
                                LogLikelihood      AIC        BIC  
                                _____________    _______    _______

    constant error model            3.9866       -3.9732    -2.8433
    equal weights                   3.9866       -3.9732    -2.8433
    proportional weights           -3.8472        11.694     12.824
    proportional error model       -3.8257        11.651     12.781
    exponential error model         1.1984        1.6032     2.7331
    combined error model            3.9163       -3.8326    -2.7027

Based on the information criteria, the constant error model (or equal weights) fits the data best since it has the largest loglikelihood value and the smallest AIC and BIC.

Display Estimated Parameter Values

Show the estimated parameter values of each model.

Estimated_Central       = zeros(6,1);
Estimated_Cl_Central    = zeros(6,1);
t2 = table(Estimated_Central,Estimated_Cl_Central);
t2.Properties.RowNames = errorModelNames;
for i = 1:height(t2)
    t2{i,1} = allResults(i).ParameterEstimates.Estimate(1);
    t2{i,2} = allResults(i).ParameterEstimates.Estimate(2);
end
t2

t2=6×2 table
                                Estimated_Central    Estimated_Cl_Central
                                _________________    ____________________

    constant error model             1.6993                0.53358       
    equal weights                    1.6993                0.53358       
    proportional weights             1.9045                0.51734       
    proportional error model         1.8777                0.51147       
    exponential error model          1.7872                0.51701       
    combined error model             1.7008                0.53271

Conclusion

This example showed how to estimate PK parameters, namely the volume of the central compartment and clearance parameter of an individual, by fitting the PK profile data to one-compartment model. You compared the information criteria of each model and estimated parameter values of different error models to see which model best explained the data. Final fitted results suggested both the constant and combined error models provided the closest estimates to the parameter values used to generate the data. However, the constant error model is a better model as indicated by the loglikelihood, AIC, and BIC information criteria.

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