Maybe I can count the muscle fibers using a manual cell counter
and then I can crop the image (imfreehand?) on one muscle fiber or a few muscle fibers that have clear divides between them. Then I can use something like this to calculate area and other parameters (<http://www.mathworks.com/help/images/examples/measuring-regions-in-grayscale-images.html?prodcode=IP&language=en>)
Are there any better, more automated ways to accomplish this? I need to count all the fibers, but do not need to find the areas or diameters on all the fibers.
Thanks again
Mohammad