Wavelength Calibration From Intensity Values (of a RGB Image)
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I am working on a project for school which involves image processing of a color image. I am taking pictures of a spectrum for a blank and a sample, and I wannt to compare them to obtain a proper wavelength scale. Each image contains a full spectrum (Blue to Red or 400-750nm wavelength). I want to use analysis to determine a proper wavelength scale for graphing Intensity and Absorbance. (Intesity vs. Wavelength and Absorbance vs. Wavelength)
I have the intensity plots vs. pixel count, which is working nicely, but I would love to have intensity or absorbance vs. wavelength. I'm just unsure on how to go about obtaining wavelegnth from the image. I have all the camera information (pixel size, CMOS size, ect...) The research I have done, hasn't came up with to much. I'm not sure if this is even possible and that is why I need help.
Any help on the subject will be a blessing!
Thanks
Answers (1)
Image Analyst
on 2 Apr 2013
0 votes
Check out this link for lots of papers published on the topic: http://iris.usc.edu/Vision-Notes/bibliography/active670.html#Photometric%20Calibration,%20Radiometric%20Calibration,%20Color%20Calibration You don't specify whether the "full spectrum" applies to the illumination or to the reflectant scene. As you know the signal is the integral over all wavelengths of (the spectral responsivity of the sensor) * (the spectral reflectance of the sample) * (the spectrum of the illumination source). Do you know any of those three spectra in advance? Or are all three unknown?
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