how to find curvature of edges of a fluorescent image?

hi I have a fluorescent image like this:

and I want to use the function LineCurvature2D to find the curvature of the entire body.(see the function in http://www.mathworks.com/matlabcentral/fileexchange/32696-2d-line-curvature-and-normals). so firstly I need to detect the edges of the image and make it a 2D line and then extract the "vertices" and "lines" in .mat files as inputs of the function.

I also used the links below for my aim but they didn't work:

http://www.mathworks.com/help/images/examples/detecting-a-cell-using-image-segmentation.html

http://www.mathworks.com/matlabcentral/fileexchange/47082-edge-detection-using-log-zip

I'd be very appreciate if someone help me to achieve what I need.

Answer 1

[Image Analyst]

I don't know why you culdn't get that code to work. But anyway, see the attached code, below the image.

[mogh]

great! I tried it and got the vertices. thanks for your help!

for calcuating "lines" should I add this at the end?

Lx=[(1:(size(x,1)-1),(2:size(x,1)] ;

Ly=[(1:(size(y,1)-1),(2:size(y,1)];

lines=[Lx Ly];

excuse me , I'm new to matlab and I've just learnt the basics.

[Image Analyst]

What do you mean by lines? And why do you need them? I plotted the boundary with no "lines" and I can make measurements of the region without any "lines" so I don't know what you want or why you think you need lines.

[mogh]

see the link again please:

http://www.mathworks.com/matlabcentral/fileexchange/32696-2d-line-curvature-and-normals

I have to have two .mat files as inputs(Vertices & Lines) to find the curvature of each coordinate.

[Image Analyst]

I got you the boundary points. I have not run that File Exchange program, so you're on your own for that, for adapting the boundary coordinates into that program.

[mogh]

Ok! thanks again for your help.

Best Regards,

mogh